FastQCFastQC Report
Fri 14 Jul 2017
HJGFVAFXX_n01_ngnz2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJGFVAFXX_n01_ngnz2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39053027
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG157950.023.89445119
TACGGGG120150.022.25250822
AGAGTAC274400.021.97643518
AGTACGG173600.021.8595820
GTACGGG173500.021.81263721
CGCAGAG277050.021.77865215
AGTGGTA304600.020.0740224
GTGGTAT315850.019.2934725
ACGCAGA315650.019.24677314
GCAGTGG325000.018.8791712
AGCAGTG329450.018.6270981
AACGCAG327900.018.4856513
TCAACGC333000.018.33714511
CAGAGTA333450.018.25018317
ATCAACG361850.016.80857510
GCAGAGT363950.016.69233116
TGGTATC374500.016.235116
GTATCAA383500.016.183528
GGTATCA384850.016.180377
GAGTACT177250.016.17385720
Produced by FastQC (version 0.11.2)