FastQCFastQC Report
Fri 14 Jul 2017
HJGFVAFXX_n01_ngnz1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJGFVAFXX_n01_ngnz1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26395668
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG116200.024.93853219
TACGGGG83150.023.19261622
AGAGTAC199150.023.16054518
GTACGGG125050.023.0633721
AGTACGG124950.023.02643420
CGCAGAG203400.022.64264915
AGTGGTA220550.021.0269534
ACGCAGA225150.020.45543114
GTGGTAT228100.020.2251195
AGCAGTG236950.019.7368851
GCAGTGG239150.019.5665262
CAGAGTA237900.019.48954617
AACGCAG238950.019.40401513
TCAACGC241600.019.09115411
GCAGAGT259500.017.78752916
TGGTATC264100.017.520456
ATCAACG267600.017.4169410
GTATCAA271800.017.211278
CAACGCA282300.016.49759312
GGTATCA285500.016.4336417
Produced by FastQC (version 0.11.2)