FastQCFastQC Report
Fri 14 Jul 2017
HJGFVAFXX_n01_ngnngnw2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJGFVAFXX_n01_ngnngnw2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18886355
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACACAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA286720.15181330648502583TruSeq Adapter, Index 5 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG64650.035.3808549
GCCGTCT70300.032.2923552
CGTCTGA77650.030.16659717
CGTATGC76550.030.01509547
CACGCAC77750.029.81759632
AGTCACG79150.029.116329
GTATGCC80750.028.49688748
TGCCGTC81250.028.1951851
GTCACGC82750.027.6412330
GAGTACG83300.026.21555319
CTCGTAT83450.025.67343545
TCTCGTA83850.025.63318444
CACGTCT93050.025.50762715
TACGGGG62150.025.47851822
ACGTCTG94100.025.039716
CGCACAC94050.024.75995834
AATCTCG86600.024.540242
AGAGTAC145450.024.28764518
CGCAGAG148650.024.02002515
ATGCCGT97150.023.97095950
Produced by FastQC (version 0.11.2)