FastQCFastQC Report
Fri 14 Jul 2017
HJGFVAFXX_n01_ngnngnw1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJGFVAFXX_n01_ngnngnw1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30210449
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG126400.023.7995819
AGAGTAC220800.021.74907518
CGCAGAG221550.021.70659315
AGTACGG139150.021.64363720
TACGGGG91000.021.15418422
GTACGGG142150.021.06565321
AGTGGTA248350.019.7574544
ACGCAGA247150.019.50011314
GTGGTAT256950.019.1633225
AGCAGTG260200.018.8626271
GCAGTGG262200.018.8074362
CAGAGTA262900.018.48932517
AACGCAG261300.018.37812413
TCAACGC265850.018.25817511
GCAGAGT291900.016.68788316
TGGTATC297100.016.5968286
TCCGATC111000.016.2582656
ATCAACG305900.016.02571310
GTATCAA315150.015.63196858
CAACGCA324150.015.17658712
Produced by FastQC (version 0.11.2)