FastQCFastQC Report
Fri 14 Jul 2017
HJGFVAFXX_n01_ngnngn2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJGFVAFXX_n01_ngnngn2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25036261
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG57700.028.81986249
GAGTACG119400.025.71494119
GTACGGG125650.024.43594221
CGTATGC69450.024.24140747
TACGGGG84350.024.21242122
AGTACGG126600.024.19788220
GCCGTCT68550.023.60421252
AGAGTAC205400.023.43011918
CGCAGAG206950.023.0713115
GTATGCC74650.023.0150348
CGTCTGA82250.022.14617517
AGTGGTA222200.021.6792144
AGCAGTG227450.021.2593441
ACGCAGA226950.021.19024314
TGCCGTC78850.021.1334551
GTGGTAT231900.020.831935
TCAACGC235950.020.2795411
GCAGTGG239450.020.1620852
AACGCAG238800.020.12426813
CAGAGTA239950.020.01332317
Produced by FastQC (version 0.11.2)