FastQCFastQC Report
Fri 14 Jul 2017
HJGFVAFXX_n01_ngnngn1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJGFVAFXX_n01_ngnngn1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27258162
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG112100.024.12753119
GTACGGG119500.022.60473821
AGAGTAC193350.022.4638418
CGCAGAG195800.022.2531515
AGTACGG122950.021.97028420
TACGGGG82950.020.71183622
AGTGGTA219600.019.9875324
ACGCAGA219550.019.95614
CAGAGTA232500.018.9185617
GTGGTAT232500.018.819185
TCAACGC235900.018.61667411
AACGCAG239750.018.38973413
GCAGTGG242050.018.348522
AGCAGTG242300.018.3322771
GCAGAGT252650.017.35509916
TGGTATC255800.017.253396
ATCAACG265550.016.68105710
GTATCAA275300.016.0902828
GGTATCA279450.016.0735177
TCCGATC104700.015.9515586
Produced by FastQC (version 0.11.2)