FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l02n01_ah9251-inp_1217.351000000ab916.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l02n01_ah9251-inp_1217.351000000ab916.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6699012
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC323630.48310109013090285TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG40300.039.19145611
GATCGGA42150.037.9641761
AGTCACG41600.037.7503928
GTCACGA41650.037.5970329
CACACGT42750.037.2611712
ACACGTC42600.037.23392513
ACGTCTG42750.037.1559115
GATCTCG41900.037.00290739
CGATCAG42600.036.9701333
CACGTCT43100.036.95858414
CACGATC43800.035.8542531
AGCACAC44500.035.6947210
CGTCTGA45200.035.29125616
GAGCACA45300.034.865699
GTATGCC45550.034.77830545
TCCAGTC45450.034.7011225
CAGTCAC45550.034.6249327
CGTATGC45700.034.4669444
TCACGAT45900.034.45894230
CTCGTAT46100.033.8767842
Produced by FastQC (version 0.11.2)