FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l02n01_ah9251-chip_1217.351000000ab8fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l02n01_ah9251-chip_1217.351000000ab8fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9116670
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1164101.2768916720688586TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG137400.041.9849411
ACACGTC137850.041.83156213
CACACGT139650.041.34071412
ACGTCTG138800.041.33475515
CACGTCT139400.041.26959614
CGTCTGA140900.040.76704416
GATCGGA144650.040.683911
AGCACAC142450.040.6386810
CATCTCG140400.040.5171539
GTATGCC141650.040.5095445
GAACTCC142650.039.96724721
CGTATGC143950.039.84490644
AGAGCAC146650.039.812338
GAGCACA147000.039.7175379
CAGTCAC143000.039.695727
TCGGAAG147950.039.6652033
TCCAGTC142600.039.53904325
CCAGTCA143450.039.4459226
CTCGTAT144950.039.38546442
AGTCACC146000.038.9108628
Produced by FastQC (version 0.11.2)