FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l02n01_ah9120-inp_1217.351000000ab8d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l02n01_ah9120-inp_1217.351000000ab8d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6006757
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC237660.3956544271725991TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC30100.037.30003733
ACTTAGG31000.036.4348732
GCACACG31000.036.43426511
ACACGTC31450.035.9129513
ACGTCTG31800.035.72993515
CACGTCT32550.034.9757914
GATCGGA32950.034.899871
CGTCTGA32700.034.6777416
CACACGT32750.034.55609512
CAGTCAC32800.034.22903427
CATCTCG32700.034.19866639
AGCACAC33750.033.6655410
GTATGCC33850.033.37001445
CACTTAG34500.033.0641131
TAGGCAT34200.033.0257635
GAGCACA35000.032.84899
AGAGCAC35200.032.7900928
TTAGGCA34600.032.5789334
GCATCTC35000.032.14365438
CGTATGC35250.032.10824644
Produced by FastQC (version 0.11.2)