FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l02n01_ah9120-chip_1217.351000000ab8ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l02n01_ah9120-chip_1217.351000000ab8ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7299989
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1341551.8377424952284176TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG152700.042.8765611
ACACGTC153600.042.6399813
GTCACGC150800.042.49175329
AGTCACG151650.042.41678228
ACGTCTG154200.042.34274715
CACACGT154900.042.267612
CACGTCT155500.042.0900414
AGCACAC156400.041.96291710
TCACGCC153850.041.86873630
GAACTCC156150.041.54020721
CACGCCA155450.041.42331731
GAGCACA159250.041.39569
CAGTCAC156000.041.2195927
CGTCTGA158450.041.15021516
GTATGCC157900.041.1284845
CCAGTCA156250.041.12483626
CGTATGC158200.041.1062644
TCCAGTC156900.041.0261625
TCGGAAG161150.041.0234453
AGAGCAC160500.041.017138
Produced by FastQC (version 0.11.2)