FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l02n01_ah8869b-1217.351000000ab84e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l02n01_ah8869b-1217.351000000ab84e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5027719
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC144820.2880431464049602TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCT21700.032.1410631
GCACACG22450.031.56838811
GTATGCC22450.031.47474545
TCACGGC22950.030.48849130
CACACGT23650.030.15687612
GATCGGA24100.029.8815751
ACGTCTG24200.029.75040215
GTCACGG23300.029.64426629
ACACGTC24400.029.3221313
CGTCTGA24400.029.13771416
CGGCTAC24250.029.1326833
CATCTCG24550.028.78240839
ACGGCTA24700.028.60192532
CACGTCT25000.028.52840414
AGTCACG24350.028.45836828
CAGTCAC25350.027.60200727
GGCTACA25850.027.32949834
AGCACAC26200.027.2217610
CGTATGC26150.027.02026744
GAGCACA26600.026.7278299
Produced by FastQC (version 0.11.2)