FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l02n01_ah8869a-1217.351000000ab824.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l02n01_ah8869a-1217.351000000ab824.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4225284
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG5900.025.16798411
CGTCTGA7450.020.23368516
CACACGT7250.020.17120412
GATCGGA7800.019.9106641
ACACGTC7700.018.99236713
ACGTCTG8200.018.1086715
CACGTCT8600.017.52801914
CACTAGC8700.017.32654831
CAGTCAC8700.016.80933827
CGTATGC8850.016.7820344
TATCTCG9150.015.73936839
TCCAGTC9800.015.61130825
AGAGCAC9600.015.4678238
GTATGCC9850.015.3067345
ACTAGCT9750.015.23021732
GAGCACA10250.014.9259359
TCACTAG10400.014.71065630
AGCACAC9800.014.69299610
TCGTATG10350.014.56796543
CCAGTCA10400.014.27798926