FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l02n01_ah8868b-1217.351000000ab80a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l02n01_ah8868b-1217.351000000ab80a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6371010
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC281520.4418765627427991TruSeq Adapter, Index 2 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC263620.4137805465695392TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC40950.035.8825545
AGATCGG50600.026.5095021
ACGTCTG69900.021.08289315
CACGTCT69500.021.07474114
GCACACG69550.021.05959111
TATCTCG69750.020.93682739
ACACGTC70500.020.90346513
CCGATGT70450.020.82248933
CGATGTA71750.020.75879734
CGTCTGA71000.020.6611916
CACACGT71400.020.63997712
CAGTCAC70450.020.59893627
CGTATGC71650.020.56989744
TCCAGTC70900.020.37299525
CCAGTCA70800.020.30643526
CTCGTAT72400.020.2952942
GTCACCG71450.020.27914829
CACCGAT72000.020.21798531
AGTCACC71950.020.20076428
ACCGATG72900.020.06096632
Produced by FastQC (version 0.11.2)