FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l02n01_ah8868a-1217.351000000ab7ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l02n01_ah8868a-1217.351000000ab7ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5243523
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC254960.48623797397284224TruSeq Adapter, Index 4 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC247050.47115269638370993TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC32850.036.51148645
AGATCGG32600.036.2444461
GCACACG56950.021.2150311
ACGTCTG57550.020.91565915
ACACGTC57900.020.86694113
CACACGT58150.020.69984812
CGTCTGA58450.020.55511316
GATCGGA59700.020.4326531
AGCACAC59950.020.3785710
CACGTCT59550.020.32654614
CGTATGC59200.020.29741144
ACTGACC59250.020.20162832
GAGCACA60700.020.0526479
CAGTCAC59600.020.04524427
TCCAGTC59950.020.04080425
CCAGTCA59650.020.02844226
AGAGCAC60950.019.9334818
CTCGTAT60100.019.91876442
TCTCGTA60100.019.88113441
ATCTCGT61250.019.80175840
Produced by FastQC (version 0.11.2)