FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l02n01_ah8583-inp_1217.351000000ab881.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l02n01_ah8583-inp_1217.351000000ab881.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6955413
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC746571.0733654493270204TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG87500.041.50092711
GATCGGA89600.040.9902571
ACACGTC89400.040.64408513
CACACGT90100.040.35328312
ACGTCTG90100.040.17877215
CGATCTC88850.040.0655138
CACGATC89350.040.03788436
CACGTCT90850.039.9458614
AGCACAC91050.039.93224710
CATCACG91000.039.68279333
GATCTCG90100.039.65979439
GTATGCC90800.039.55230345
CGTCTGA91500.039.5148416
GAGCACA92450.039.3275389
CAGTCAC91550.039.2963727
CGTATGC91850.038.8788344
CTCGTAT92350.038.69492342
CCAGTCA93250.038.53172326
TCGGAAG95600.038.4594763
TCCAGTC93700.038.41870525
Produced by FastQC (version 0.11.2)