FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l02n01_ah8583-chip_1217.351000000ab867.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l02n01_ah8583-chip_1217.351000000ab867.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8000271
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC3898284.8726849377977315TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG420950.043.9774511
ACACGTC423100.043.8177913
CACACGT423650.043.7290412
GATCGGA429650.043.695121
CACGTCT424200.043.57156814
ACGTCTG423300.043.5525915
AGCACAC427100.043.48643510
GAGCACA429950.043.292379
TCGGAAG433050.043.2427143
CGTCTGA427200.043.1760616
AGAGCAC432900.043.0805138
GTCACAC422100.043.0527629
GTATGCC425700.042.93797345
GAACTCC428500.042.9295521
ATCGGAA437700.042.9126472
CACACAG427000.042.8959431
GATCTCG423950.042.80575639
TCCAGTC426350.042.69220425
CGGAAGA439500.042.657964
CAGTCAC426200.042.65443827
Produced by FastQC (version 0.11.2)