FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l02n01_ah7797k-1217.351000000ab7c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l02n01_ah7797k-1217.351000000ab7c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12393172
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT990440.7991820011858143TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG923720.7453459049870363TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG122250.039.9306221
TCGTATG120300.036.7180845
GCACACG212800.022.66811212
GATCGGA218600.022.3071732
CACACGT217600.022.21977813
ACACGTC216900.022.13589314
ACGTCTG217800.022.0961616
CACGTCT218150.022.00905615
AGCACAC222600.021.80154211
GAGCACA224250.021.58092910
CAGTCAC220950.021.57740228
CGTCTGA224450.021.51166717
CCAGTCA222600.021.4275727
TCCAGTC223400.021.38113626
AGAGCAC226750.021.3231479
GTCACAT226200.021.30536830
TCGGAAG229100.021.3036734
AGTCACA226850.021.21456729
TCACATG226000.021.17489431
GAACTCC225850.021.14928222
Produced by FastQC (version 0.11.2)