FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l02n01_ah7797j-1217.351000000ab7ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l02n01_ah7797j-1217.351000000ab7ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8323399
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG121910.14646660577007062TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT119040.14301849520850798TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG22050.028.475451
TCGTATG28850.021.44977445
CGTCCCG28800.020.85855335
GTCCCGA30050.019.99089236
GCACACG32300.019.2947912
CCCGTCC32350.019.12598633
ACCCGTC32750.018.96108632
ACGTCTG32600.018.63434816
GATCGGA34000.018.5975382
TCACCCG32850.018.56080231
CCCGATC32600.018.4288538
ACACGTC34000.018.26387814
CACCCGT33450.018.22798332
CACACGT34100.018.21031813
CCGTCCC34250.017.8679134
CACGTCT35550.017.40427415
CCGATCT35650.016.9794440
TCCCGAT35800.016.97013537
CGTCTGA36200.016.8433616
CGATCTC35600.016.7504541
Produced by FastQC (version 0.11.2)