FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l02n01_ah6408k-1217.351000000ab79f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l02n01_ah6408k-1217.351000000ab79f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13336481
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG169340.1269750243711216TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG27850.030.13366332
CGTCCGC28900.028.96098933
GCACACG29800.028.53922811
AGTCACG30850.027.05736228
ACACGTC31450.026.6842413
ACGTCTG32200.026.20246115
CCGCACA32000.026.15539436
CACACGT32950.025.6743312
GTCACGT33150.025.11220729
CATCTCG33450.025.02413641
CACGTCT34100.024.9404414
AGCACAC34700.024.83338710
TCACGTC34900.024.23980330
CAGTCAC38050.022.35135327
CACGTCC37900.022.2617231
GAGCACA38950.022.0659479
TCCAGTC39950.021.175725
CCAGTCA39750.021.05583626
CGTCTGA40650.020.75585616
GTCCGCA41200.020.42408834
Produced by FastQC (version 0.11.2)