FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l02n01_ah6408j-1217.351000000ab775.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l02n01_ah6408j-1217.351000000ab775.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10473043
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG316020.30174611142148466TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT112290.10721812180089396TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG55900.029.5428411
TCGTATG56300.029.2960645
AGTCACG57350.028.52127828
ACACGTC58950.028.16713
ACGTCTG60100.027.62815915
CACACGT60500.027.40817512
GATCGGA62400.027.3751071
GTCACGT60150.027.30581929
TCACGTG61300.027.0137830
CACGTCT61650.026.93340714
CACGTGA62150.026.71672631
CGATCTC60350.026.65865740
CGTCTGA62450.026.62453716
GATCTCG61300.026.46575541
AGCACAC62750.026.46126710
CAGTCAC63050.025.87145627
TCCAGTC64450.025.30947125
GAGCACA67350.024.9546249
CCAGTCA66150.024.79508826
AGAGCAC68350.024.4578518
Produced by FastQC (version 0.11.2)