FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l01n01_ah8643i-1217.351000000ab7fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah8643i-1217.351000000ab7fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12268861
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC310160.2528026032734416TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC49050.031.92611545
GCACACG55500.028.70132411
ACACGTC58150.027.2385913
CACACGT59000.026.99870512
CACGTCT59150.026.93023714
ACGTCTG59550.026.82523715
GATCGGA60250.026.706821
CATCTCG60700.025.6904539
CAGTCAC62100.025.25271827
AGCACAC64600.024.93688210
CGTCTGA64000.024.9248916
CGTATGC63000.024.8956244
GAGCACA65400.024.7350529
CCAGTCA64250.024.5477626
AGAGCAC68450.023.6329048
CTCGTAT66100.023.62582242
AGTCACC66850.023.59302328
TCCAGTC67550.023.38184225
ATCTCGT67400.023.27019740
TCGGAAG69550.023.2608343
Produced by FastQC (version 0.11.2)