FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l01n01_ah8643a-1217.351000000ab7d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah8643a-1217.351000000ab7d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11943849
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG22500.015.9032721
GCACACG24000.015.09287411
GTATGCC23700.014.05075845
GATCGGA26550.013.6468741
ACGTCTG26950.013.44100415
ACACGTC26900.013.21484713
CACGTCT28100.013.05084414
CACACGT27550.012.98472712
CGTCTGA29050.012.70176616
CAGTCAC30950.011.84936827
TCCAGTC31150.011.77328825
AGCACAC32350.011.47537710
CCAGTCA31250.011.44762326
CGTATGC30900.011.35932445
GAGCACA34100.010.8204869
TCGGAAG36600.010.5736883
AGTCACC34700.010.56868428
ATCTCGT32100.010.44541841
AGAGCAC34700.010.3091998
TCGTATG34600.010.21116844
Produced by FastQC (version 0.11.2)