FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l01n01_ah8630i-1217.351000000ab7b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah8630i-1217.351000000ab7b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10467843
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT424750.40576649840850687TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG260730.2490771021307828TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG62450.033.6209341
TCGTATG47950.026.93425845
GCACACG83950.024.84391412
ACACGTC85000.024.4840814
ACGTCTG85700.024.25807416
CACACGT86700.024.10780113
CACGTCT86350.024.04941415
AGCACAC89350.023.41797811
CAGTCAC89400.023.0274928
CGTCTGA90900.022.89512317
GTCACAG90500.022.87190230
TCCAGTC90150.022.81117626
GAGCACA92500.022.7664410
GATCGGA92800.022.744372
CCAGTCA90650.022.68535627
AGTCAAC92500.022.54817835
CTCGTAT92200.022.52436345
CACAGTC93300.022.28259832
AGTCACA93000.022.20868529
ATCTCGT94550.022.01538343
Produced by FastQC (version 0.11.2)