FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l01n01_ah8584-inp_1217.351000000ab966.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah8584-inp_1217.351000000ab966.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7767647
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG3756524.836110600803564TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA428450.043.8795621
GCACACG422150.043.80401211
ACACGTC422600.043.49117313
CACACGT425650.043.43853812
GAGCACA428900.043.382169
TCGGAAG433000.043.3119133
CACGTCT423050.043.26408814
AGCACAC429150.043.2625210
ACGTCTG423250.043.21790315
AGAGCAC433000.043.1272628
CGTCTGA425100.043.0827516
ATCGGAA436950.043.036712
AGTCACG424700.042.85761328
GAACTCC428350.042.68288421
GTCACGT426650.042.67227629
TCGTATG425350.042.58802445
TCACGTG428300.042.50263630
GATCTCG422850.042.49354641
TCCAGTC428750.042.49005525
CAGTCAC428950.042.48073227
Produced by FastQC (version 0.11.2)