FastQCFastQC Report
Fri 8 Dec 2017
HJCFYBCXY_l01n01_ah8584-chip_1217.351000000ab94d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah8584-chip_1217.351000000ab94d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10353873
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC8293898.010422766437255TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG910050.044.4314611
GATCGGA923850.044.2858661
CACACGT913400.044.2192412
AGCACAC917850.044.1788910
ACACGTC912500.044.17655613
CACGTCT912750.044.09297614
GAGCACA922300.044.075519
ACGTCTG913750.044.0722315
TCGGAAG927500.044.0543373
AGAGCAC926650.043.9220168
CGTCTGA917350.043.88946516
ATCGGAA935600.043.7486722
CGGAAGA934450.043.7043724
GAACTCC913900.043.6741921
GTCTGAA925800.043.47429717
GTATGCC916500.043.34904545
TCTGAAC927650.043.29300318
CCGATGT917450.043.29022633
ACTCCAG920300.043.27470823
TCCAGTC919300.043.26548825
Produced by FastQC (version 0.11.2)