FastQCFastQC Report
Thu 7 Dec 2017
HJCFYBCXY_l01n01_ah7797top2-0817-1217.351000000ab85b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah7797top2-0817-1217.351000000ab85b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7195969
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC373210.5186375872380774TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG45300.037.6974311
ACGTCTG46050.037.0834615
GATCGGA46800.037.0259861
ACACGTC47650.035.83827213
CACACGT47850.035.7825212
CACGTCT47800.035.77287714
CGTCTGA47700.035.75378416
GAACTCC48100.035.5042221
GTCACAC48200.035.19666729
GAGCACA49700.034.8127339
GTATGCC49100.034.78087645
TCCAGTC48850.034.77439525
CCAGTCA49200.034.5727526
AGAGCAC50350.034.2292568
AGCACAC50600.034.0601410
CAGTCAC49800.033.97548727
ATCTCGT50500.033.5509640
CGTATGC51700.033.1206244
TCGGAAG53200.032.6940883
TCGTATG52550.032.32798443
Produced by FastQC (version 0.11.2)