FastQCFastQC Report
Thu 7 Dec 2017
HJCFYBCXY_l01n01_ah7797red1-1217.351000000ab89e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah7797red1-1217.351000000ab89e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6306490
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC169980.26953186320758454TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22750.036.39411511
ACGTCTG24150.034.28431315
ACACGTC24750.033.72589513
CACACGT25200.033.2129312
CACGTCT25150.032.9211214
GATCGGA25100.032.905671
CACTAGC25800.031.48152231
AGCACAC26450.031.38812810
GAACTCC26550.031.0164221
CGTCTGA26550.031.01568216
TATCTCG26750.030.6189739
CGTATGC27050.030.28034844
GTATGCC27200.030.19369545
GAGCACA27650.030.1886399
CAGTCAC26900.030.1107727
TCGGAAG28650.029.059633
ATCTCGT28700.028.61721840
CCAGTCA28450.028.4702926
TCCAGTC28400.028.44118925
CTCGTAT28950.028.2153142
Produced by FastQC (version 0.11.2)