FastQCFastQC Report
Thu 7 Dec 2017
HJCFYBCXY_l01n01_ah7797i3h34c-0817-1217.351000000ab817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah7797i3h34c-0817-1217.351000000ab817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7216459
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC12150.017.03637113
GCACACG11950.016.38011211
CACGTCT13100.015.11391414
CACACGT13750.015.05395712
CGATCTC13150.014.54527438
GATCTCG13350.014.1588139
CACGATC13750.013.9087436
ACGTCTG14100.013.88253115
CGTCTGA14800.013.37794916
GATCGGA14850.013.0321411
CAGTCAC15300.012.64682727
AGCACAC15900.012.59384710
GAACTCC16100.012.29808221
CGTATGC16350.012.111464544
CATCACG16300.011.87086633
AGTCACA17600.011.76090428
CCAGTCA16650.011.75654126
TCACGAT17000.011.51441635
TCCAGTC16700.011.45188625
GTATGCC16400.011.38722345