FastQCFastQC Report
Thu 7 Dec 2017
HJCFYBCXY_l01n01_ah7797hop1-1217.351000000ab874.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah7797hop1-1217.351000000ab874.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6177150
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC192650.3118752175355949TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG26200.037.183811
AGTCACG26700.036.57203328
GATCGGA27900.035.571321
ACACGTC27700.035.25145713
ACGTCTG27650.035.1527515
CACACGT28350.034.52258712
CGATCAG28600.034.2213733
GATCTCG28450.034.0882239
CACGTCT28900.033.63202714
GTCACGA29100.033.47846629
AGCACAC30000.032.77383810
CACGATC30000.032.54963731
GAACTCC30300.032.52465421
CGTCTGA30850.031.8710916
CGTATGC30300.031.8586644
GAGCACA30800.031.8495229
CAGTCAC31900.030.96360627
GTATGCC31350.030.93291945
TCCAGTC32650.030.11451725
AGAGCAC33350.029.953968
Produced by FastQC (version 0.11.2)