FastQCFastQC Report
Thu 7 Dec 2017
HJCFYBCXY_l01n01_ah7606top2-1217.351000000ab8b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah7606top2-1217.351000000ab8b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6196638
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC104920.16931762029668346TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG14950.033.7106411
GTCACGG15200.032.7121329
CACGGCT15350.032.3929931
TCACGGC15650.031.77152830
AGTCACG16600.030.08881228
GATCGGA17600.029.2818581
ACGTCTG17600.029.14646115
ACGGCTA17500.028.4132832
CATCTCG17950.028.2075839
CACACGT18200.028.18536412
CACGTCT18200.028.06174314
CGGCTAC17850.027.98220433
ACACGTC18100.027.8438713
AGCACAC19200.026.36583110
CGTCTGA19550.026.35435516
GAACTCC19350.026.04580321
CAGTCAC19400.025.86227827
GTATGCC19450.025.44938345
CCAGTCA20350.025.0971926
GGCTACA20200.024.61566534
Produced by FastQC (version 0.11.2)