FastQCFastQC Report
Thu 7 Dec 2017
HJCFYBCXY_l01n01_ah7606red1-1217.351000000ab8ee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah7606red1-1217.351000000ab8ee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6498882
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG9200.030.32489811
CACACGT11350.025.76991312
AGTCACG11150.025.62679528
ACGTCTG11450.024.9553515
ACACGTC11850.023.9231113
GATCGGA12150.022.9675391
CACGTCT12850.021.53611814
GTCACGT13700.021.18529729
AGCACAC13300.021.14578410
CGTTTCG13350.020.7301633
GAACTCC14150.019.87569621
CGTCTGA14550.019.79303616
CAGTCAC14750.019.37211827
TCCAGTC14900.018.87509525
GTTTCGG15550.018.37604934
CCAGTCA16350.017.20115926
CTCGTAT16750.016.92705244
ACTCCAG17250.016.69512423
AGAGCAC17150.016.5299638
TCGGAAG17300.016.518093
Produced by FastQC (version 0.11.2)