FastQCFastQC Report
Thu 7 Dec 2017
HJCFYBCXY_l01n01_ah7606i3h34c-0817-1217.351000000ab831.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah7606i3h34c-0817-1217.351000000ab831.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6647743
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC9050.016.15988333
GCACACG9650.015.38781711
ACTTAGG10050.014.99968732
CGTCTGA12450.013.19229716
ACACGTC11400.013.02565313
ACGTCTG11600.012.99522315
CACGTCT11450.012.96877214
CACACGT11750.012.82913212
GATCGGA12300.012.0750981
CATCTCG13100.011.68107839
AGCACAC13100.011.335299510
CAGTCAC13350.011.29164427
GCATCTC14600.011.25162738
GAACTCC14600.011.24984921
GTATGCC13200.011.07931445
TAGGCAT13900.010.84509735
TTAGGCA14250.010.57872734
GAGCACA14500.010.5511869
CCAGTCA14650.010.44323326
AGTCACT15550.010.41747628