FastQCFastQC Report
Thu 7 Dec 2017
HJCFYBCXY_l01n01_ah7606hop1-1217.351000000ab8c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah7606hop1-1217.351000000ab8c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4805277
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG6300.024.99884211
AGTCACG6900.022.49919328
CACGTGG7000.022.178731
ACACGTC7300.021.8825513
GATCGGA7450.021.7516571
CACACGT7500.021.29901512
ACGTGGC7500.020.70011932
GTCACGT7750.020.32185229
TCACGTG7750.020.32185230
CACGTCT7900.019.65099114
CGTGGCC8100.019.16657633
ACGTCTG8400.019.01717615
GGCCTTA9550.016.72767336
GAACTCC10350.016.30393421
CGTCTGA9800.016.30043616
TCGGAAG11850.015.3806363
CTCGTAT10200.015.22178544
TATCTCG10500.015.21516441
AGCACAC10150.015.073194510
TCTCGTA11550.014.80635843
Produced by FastQC (version 0.11.2)