Basic Statistics
Measure | Value |
---|---|
Filename | HJCFYBCXY_l01n01_ah119spikeb-1217.351000000ab909.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8130560 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG | 366731 | 4.510525720245592 | TruSeq Adapter, Index 15 (97% over 40bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCACACG | 41280 | 0.0 | 43.842804 | 11 |
GATCGGA | 42025 | 0.0 | 43.800694 | 1 |
ACACGTC | 41460 | 0.0 | 43.71216 | 13 |
CACGTCT | 41495 | 0.0 | 43.59395 | 14 |
ACGTCTG | 41595 | 0.0 | 43.548645 | 15 |
CACACGT | 41630 | 0.0 | 43.51744 | 12 |
TCGGAAG | 42360 | 0.0 | 43.396908 | 3 |
AGCACAC | 41830 | 0.0 | 43.395424 | 10 |
GAGCACA | 42045 | 0.0 | 43.32871 | 9 |
CGTCTGA | 42110 | 0.0 | 43.021397 | 16 |
GAACTCC | 41670 | 0.0 | 42.95891 | 21 |
AGAGCAC | 42545 | 0.0 | 42.909397 | 8 |
CGGAAGA | 42885 | 0.0 | 42.844128 | 4 |
ATCGGAA | 43000 | 0.0 | 42.83028 | 2 |
CAGTCAC | 41815 | 0.0 | 42.798916 | 27 |
TCGTATG | 41860 | 0.0 | 42.758286 | 45 |
TCCAGTC | 41770 | 0.0 | 42.656498 | 25 |
GTCTGAA | 42650 | 0.0 | 42.608574 | 17 |
CCAGTCA | 41855 | 0.0 | 42.602123 | 26 |
TCACATG | 42160 | 0.0 | 42.4906 | 30 |