FastQCFastQC Report
Thu 7 Dec 2017
HJCFYBCXY_l01n01_ah119spikeb-1217.351000000ab909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah119spikeb-1217.351000000ab909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8130560
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG3667314.510525720245592TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG412800.043.84280411
GATCGGA420250.043.8006941
ACACGTC414600.043.7121613
CACGTCT414950.043.5939514
ACGTCTG415950.043.54864515
CACACGT416300.043.5174412
TCGGAAG423600.043.3969083
AGCACAC418300.043.39542410
GAGCACA420450.043.328719
CGTCTGA421100.043.02139716
GAACTCC416700.042.9589121
AGAGCAC425450.042.9093978
CGGAAGA428850.042.8441284
ATCGGAA430000.042.830282
CAGTCAC418150.042.79891627
TCGTATG418600.042.75828645
TCCAGTC417700.042.65649825
GTCTGAA426500.042.60857417
CCAGTCA418550.042.60212326
TCACATG421600.042.490630