FastQCFastQC Report
Thu 7 Dec 2017
HJCFYBCXY_l01n01_ah119spikea-1217.351000000ab923.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJCFYBCXY_l01n01_ah119spikea-1217.351000000ab923.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8332036
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG1645581.9750034685399824TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG185850.043.08519411
CGTCCCG182350.042.9384635
GATCGGA191050.042.7235531
GTCCCGA184500.042.4990736
CACACGT189550.042.2797812
ACGTCTG188700.042.25637415
ACACGTC189650.042.22189313
CCCGATC185550.042.0946238
ACCCGTC186750.042.08341232
CCCGTCC186600.041.99667433
TCACCCG186950.041.9651830
CACGTCT190850.041.87389814
AGCACAC192150.041.84819410
CACCCGT187550.041.8439331
CCGTCCC187350.041.78051434
CGTCTGA191550.041.6863916
CGATCTC187200.041.58009340
TCCCGAT189600.041.40336637
TCGGAAG197250.041.3503953
CCGATCT188250.041.3354839
Produced by FastQC (version 0.11.2)