FastQCFastQC Report
Fri 15 Nov 2024
HJ7W5DRX5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ7W5DRX5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995952679
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCG14326093014.384310923671906Illumina Single End PCR Primer 1 (100% over 8bp)
ATGCCTCA413305484.149850577388728No Hit
AATCTCTC284342392.8549789161217767No Hit
TTGGTGCT261771242.628350176866184No Hit
AACCGTGA236111732.370712333813603No Hit
CGTAAGGA224743712.2565701638119697No Hit
CAGTTCCT224322842.2523443606300093No Hit
CTGACGTG222490152.233942984353376No Hit
TTCACGCA218565802.1945400078591484No Hit
GCATTGTC218114902.190012684327545No Hit
TCATTACA213734942.1460350928982237No Hit
CCATCTTA203753732.045817379642793No Hit
CATGGCCA199326552.001365669302045No Hit
GGTCTATG194595841.9538663242051484No Hit
GGAGTTCC182966391.837099230293852RNA PCR Primer, Index 1 (100% over 8bp)
CACGACAT173947841.746547237311061No Hit
GTTAACCG170732621.7142643782175115No Hit
AGTTGGCT168109981.6879314002025994No Hit
CCTGAACT164236101.649035174692271No Hit
CCACGCGT150670701.5128299082571173No Hit
TAACCGCG149730411.503388797049463No Hit
GGCTAACA148508841.4911234552741235No Hit
GTCTGAGG141450901.4202572369404711No Hit
ATGTCAAT137562061.3812108034903936RNA PCR Primer, Index 15 (100% over 8bp)
CACCTACA136828191.3738422807134194No Hit
GGACGTAT131791521.323270902110802No Hit
TTCTTCCG125029091.2553717926190748No Hit
GAGGCTAT119836831.2032381911992427No Hit
ACCTAGTA119741831.2022843306192865No Hit
TGAACCGA116005091.1647650781609074No Hit
GTTAGTGC108411021.0885157727458656No Hit
TGGCGGTC94791610.9517682114694125No Hit
TCAGGCAA81495590.8182676920135077No Hit
CCAATGCT72258240.725518807505512No Hit
CTACACTA70623830.7091082888688127No Hit
TTCTCTGC70129610.7041460049127495No Hit
AGCTCTAG70051500.7033617307032717No Hit
GGGGGGGG69305880.6958752304335134No Hit
CGTACGCA67825340.6810096647172129No Hit
GAGTAGCC64722830.6498584858969991No Hit
TTCAGCAC64558920.6482127249742555No Hit
TCCAGAGA63729070.639880501792395No Hit
TAGCGACG61981810.6223368971930844No Hit
CACGTCGT61825610.6207685495868825No Hit
TGTTACGG58871430.5911066985542995No Hit
TTCCGTCT57956660.5819218244203347TruSeq Adapter, Index 14 (100% over 8bp)
TTATGGCC57412350.5764566049226924No Hit
TGCAACCT56992820.5722442561952283No Hit
GTGTATAT56439770.56669128152423No Hit
GTCGGTAA53318200.5353487281497639No Hit
GTTCCTAG50618650.5082435246905942No Hit
ACTAGAGC50238650.504428082370769No Hit
CTCTAGCT49293220.4949353622854204No Hit
TCAGTGTC46121180.4630860579270554No Hit
GAAGGACT41963390.42133919497193295No Hit
CGATCTCG41707970.4187746152947493Illumina Paired End PCR Primer 2 (100% over 8bp)
GAAGTCAG41444070.41612489101000755No Hit
TAGCACAG40384130.4054824175034927No Hit
AGAGGATA32687800.32820635647891055No Hit
TGGTTCAC31820110.31949419556709685No Hit
TTCCACGA30058500.3018065078170245No Hit
GCGATCTA29106470.2922475195229632No Hit
AGTGTAGA27734850.27847558006317685Illumina Single End PCR Primer 1 (100% over 8bp)
ATAGAGAG26907790.2701713702604539ABI Solid3 Adapter B (100% over 8bp)
TCTACTCT26093610.26199648387109764No Hit
CTCCTTAC25987700.2609330799340116No Hit
CATACTAG25962080.2606758387965539No Hit
TATGCAGT24845260.24946225381858733No Hit
CCTATCAC24355640.24454615679586922No Hit
GCATCCAT24075820.24173658555920208No Hit
CTGTTGGA23892750.2398984460184378No Hit
TCTCAGTG23509940.23605478950672115No Hit
TTGTGCGT23240070.23334512261500728No Hit
TCTCGAAC22919300.23012438726519052No Hit
GGTACCTT22815130.2290784540376742No Hit
CGAGAGTT22363960.22454841953389637TruSeq Adapter, Index 13 (100% over 8bp)
GTTGTGAC21664370.21752408981672108No Hit
TCCTCAAG21417660.21504696409376292No Hit
GCTCACAA20995790.21081112027411894No Hit
GAGCTAAC20565860.20649434891474397No Hit
TACTCCTT19788650.19869066490055598ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
ACGCTATC18644250.18720015913527155No Hit
TGATCTCG18021780.18095016339626716Illumina PCR Primer Index 5 (100% over 8bp)
AGGCTTAG16583240.16650630446268422No Hit
GTTCGACA15722090.15785980932132218No Hit
TGCTGCTT14742640.14802550674197243No Hit
GACTTCGA14684690.14744365178819907No Hit
TCACGCGT14247090.14304986873778971No Hit
GTAGATCG13584810.13640015521259521No Hit
GTGGTGCT13389660.13444072476861121No Hit
GTGACGCA12752130.12803951702608957No Hit
CACTAGAC12687230.12738787964041412No Hit
GACCATTG12581630.12632758830101004No Hit
CCTGGCCA11442360.11488859100704321ABI Solid3 GAPDH Forward Primer (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)