FastQCFastQC Report
Fri 15 Nov 2024
HJ7W5DRX5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ7W5DRX5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995952679
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGG15669374215.733050907331311No Hit
ACTGAGCG605628566.080896941891754No Hit
CGATCAGT538982015.41172308046977No Hit
CCTAAGAC502255155.042961985947929No Hit
TAGCGCTC497982985.000066674854539No Hit
ATGCGCAG313302903.1457609041684194No Hit
ACTCGCTA302092153.0331978252553102No Hit
ATCTCAGG294546862.957438302146482No Hit
CGGAGCCT281346792.8249011818763328No Hit
GCTACGCT255447362.5648543890306663No Hit
GGAGCTAC251176462.5219718295471343No Hit
TACGCTGC236449682.3741055673188263No Hit
CGAGGCTG215402642.1627798643634155No Hit
CAGAGAGG202465632.032884034242354No Hit
GCTCATGA186420351.87177919122802No Hit
CTCTCTAC177529721.7825115966177345No Hit
TAGGCATG150289741.5090048269251155No Hit
ACAGATTC148275451.4887800708451129No Hit
AAGGACAC147057661.476552682680218No Hit
AGCCATGC146538251.471337475060901No Hit
TCTTCACA133176111.3371730686413466No Hit
TTCACGCA133031531.3357213932450298No Hit
AGCACCTC131056621.3158920374770133No Hit
GCGTAGTA129097391.2962201189078784No Hit
TCCGTCTA128344991.2886655431146243No Hit
TGGCTTCA125971801.2648372021699237No Hit
GGACTCCT117564941.180426966851906No Hit
AGGCAGAA108367941.0880832220744496No Hit
ACACGACC108111031.085503681847117No Hit
TCCTGAGC108019471.084584361060793No Hit
AATCCGTC104701161.0512664126284257No Hit
TATCAGCA94923580.9530932744245373No Hit
CGTACTAG92264290.9263923070385155No Hit
ATCATTCC90755030.9112383742079377No Hit
AAGAGGCA88622940.8898308310087893No Hit
TAAGGCGA85494340.8584176919514065No Hit
GATCTACG52588350.5280205687362782No Hit
GACATAGT52006180.5221752106959291No Hit
ACGCTACT51916780.5212775776870018No Hit
GTAACGAG47654280.47847935956001375No Hit
GTAGAGGA46692190.468819362450854No Hit
TGAGTACG46120260.46307682054038635No Hit
TAGTCTCC45187420.4537105120834762No Hit
ACTCACTG41853040.42023121060353114No Hit
CGAGAGTT41844020.42014064405162366TruSeq Adapter, Index 13 (100% over 8bp)
CTGCGTAG41046540.412133436311586No Hit
ACTACGAC32927860.33061671196127185No Hit
CGAGCGAC32334740.3246614089382855No Hit
GTCTGCTA32152550.32283210515868294No Hit
TGCTCGTA32042220.3217243216030347No Hit
CGTAGCGA28812940.2893002911436518No Hit
AACGCTGA22273710.22364225198293783No Hit
GTCTATGA11134980.1118022997958119No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)