FastQCFastQC Report
Sat 2 Nov 2019
HJ7T5BGXC_n02_Pilot_SS2_PEG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ7T5BGXC_n02_Pilot_SS2_PEG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352804264
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33618540.9528949457368238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC1909600.014.98478443
TAGATCT1886150.014.86762244
GGACCAT769200.014.612046
CAACGCA2211550.014.59828954
GTCATAT1543650.014.3381871
ATACCGG617500.014.2202286
ATCAACG2340000.013.3549832
CCGGCTT941700.013.3182814
GCATAGG1276500.013.2889861
AACGCAG2422950.013.1395465
CGGCTTG953150.012.95069615
CCTCGTT1165150.012.74595312
TCAACGC2489300.012.6296233
GTATCAA2402950.012.537741
TGGACCA982400.012.4818985
CATAGGA1433200.012.3380052
TCAGGGG653550.012.13948518
GTTATAC955650.012.0260673
CGTTTAA1246650.011.81286415
GACGCTT1040500.011.75547621
Produced by FastQC (version 0.11.5)