FastQCFastQC Report
Sat 2 Nov 2019
HJ7T5BGXC_n01_Pilot_SS2_PEG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ7T5BGXC_n01_Pilot_SS2_PEG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352804264
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG45107201.2785333002664616No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACAC6192200.013.9409521
CCACACT6230100.013.9182282
TCTCGAC7080250.013.9083362
CACACTT6234600.013.8865183
CTCCTAC4219450.013.8807824
CTCTAGT5222300.013.8700081
TCTCCTA4229150.013.8617053
CCTACGT4218000.013.8465636
AATCCTT3633050.013.8382272
TCCTTAC3632900.013.8361994
CGACGCT7104950.013.8304365
TCCTACG4229500.013.8276095
TCAACCG3395950.013.8270681
AGACATC4466850.013.8263682
GGTCCAA4881950.013.8230743
GTCTCCT4243400.013.8210362
ACACTTG6261400.013.820534
TCGACCA3500850.013.8195342
TCCACCT2257400.013.817873
CTTCAGC4273050.013.8142181
Produced by FastQC (version 0.11.5)