FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30985641
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1388960.44825924369290926No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409840.13226771716615449No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314680.10155671783585178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG984950.047.560631
TGTGTTT1190150.041.542154
TTTGTGT1181500.040.0711142
TTGTGTT1261000.039.1938973
ACTTTGT759500.038.9482651
GTGTTTG1752350.032.2717931
GATTTTT405650.029.4436327
GTTTGAT1117400.026.9439853
TGATTTT454750.026.8201276
TGTTTGA2261850.025.2018762
TTTGATC234250.024.654374
CCTTGTG45750.023.597191
CTTGTGT80500.023.1354522
TTGAGGG137050.022.2811819
TTGATCG70450.022.1121715
GGTAACT115500.021.6997221
TGATTCC96350.020.9988946
TTTGATT959350.020.8373154
GTTTGAG1081900.019.5553467
TTGATTG97350.019.514735
Produced by FastQC (version 0.11.5)