FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Mock_uncut_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Mock_uncut_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10475825
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297550.2840349089451189No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240000.22909890151849616No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107920.1030181393828171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT317450.066.866391
TTTGTGT678950.045.311962
CTTTGTG690250.044.5453221
TGTGTTT815450.040.685494
TTGTGTT875600.037.5761263
GTGTTTG1222000.032.9448281
GTTTGAT724850.031.2566743
GATTTTT279500.030.1108727
TTTGATT500750.028.0574174
TTGATTT353150.027.3568695
TGATTTT317650.027.154496
TGTTTGA1623500.024.821332
CTTGTGT79450.023.7939132
CCTTGTG49500.022.1705671
TTTGAGT211250.022.1018893
ATTTTTT382450.021.943378
GTTTGAC334800.021.9329283
TTTGATC196200.021.4247024
CTTCCGA28300.021.3151194
TGATTCC88900.021.2645366
Produced by FastQC (version 0.11.5)