FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Mock_uncut_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Mock_uncut_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16725797
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT422710.25272936171591703No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324370.19393395722786783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT561200.070.780531
TTTGTGT1182800.044.6285482
CTTTGTG1194550.044.0824241
TGTGTTT1384400.040.1770174
TTGTGTT1541000.036.0418433
GTGTTTG2114100.034.0365641
GTTTGAT1271650.031.4580143
GATTTTT464150.031.2197847
TTTGATT846500.029.474
TTGATTT587700.028.3848365
TGATTTT531400.028.0580396
TGAGTCG77800.025.2759975
TGTTTGA2851600.025.2624362
TTTGAGT347350.024.4502813
CTTGTGT104250.023.5107442
TTGAGTC223200.023.323774
TTTGATC320450.022.8711514
ATTTTTT640150.022.473138
GTTTGAC564150.022.2192043
TTGATCG96700.021.6129365
Produced by FastQC (version 0.11.5)