FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Mock_4h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Mock_4h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9461402
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188280.19899799205234064No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155910.1647853034888487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT503800.067.086931
CTTTGTG1036600.046.6159631
TTTGTGT1045900.046.2830122
TGTGTTT1171250.043.116354
TTGTGTT1280550.039.406943
GTGTTTG1622050.031.1425725
GGTAACT96850.030.4140531
GTTTGAT703950.028.8858133
CCTTGTG42250.027.551
CTTGTGT70600.027.4505122
TTGAGGG144200.026.4482579
GATTTTT202950.026.2573327
GTTTGAC450300.025.2820857
TTTGATT434150.025.0756034
TAACTTT134500.025.008821
TTGATCG66200.024.9690745
GTTTGAG1031500.024.9238767
TTTGACG123250.024.6626558
TGTTTGA2057900.024.5398526
TTTGATC209650.024.4230844
Produced by FastQC (version 0.11.5)