FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Mock_4h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Mock_4h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8593441
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273540.318312536270395No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132700.15442009783973615No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99330.11558815612977387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT314850.069.627731
CTTTGTG617650.041.454731
TTTGTGT629450.040.7355732
TGTGTTT749850.035.59954
GTGTTTG1148400.034.3559951
GTTTGAT650150.034.0529753
GATTTTT251000.033.7381827
TTGTGTT818750.032.69713
TTTGATT456050.031.7144244
TGATTTT287400.030.0465186
TTGATTT323650.029.7485085
TGTTTGA1451300.027.2247522
TAACTTT80750.024.4770571
TTTGATC161550.023.991934
ATTTTTT354200.023.894798
GTTTGAC278550.022.7820533
TTGATCG50300.021.7193535
TGATTCC76950.020.8021556
TGAGTCG37950.020.0260495
CTTCCGA26150.019.2537864
Produced by FastQC (version 0.11.5)