FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Mock_30min_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Mock_30min_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10107100
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187980.18598806779392704No Hit
CTTTGTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT139820.1383383957811835No Hit
GTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA139160.1376853894786833No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124260.12294327749799647No Hit
CTTTGTGTTTGAGCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGT122400.12110298700913219No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC107420.10628172274935442No Hit
ACTTTGTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCA106540.10541104767935411No Hit
GTGTTTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAAC105000.10388736630685359No Hit
GTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGC103130.10203718178310296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT718750.065.532591
TTTGTGT1479900.045.6645472
CTTTGTG1487850.045.36591
TGTGTTT1674400.042.0097124
TTGTGTT1748550.040.111613
GGTAACT189100.036.1036871
CCTTGTG39050.033.822841
GTAACTT201750.033.6918072
CTTGTGT72150.033.1812252
GTTTGAT892750.030.8644353
TAACTTT216650.030.7160073
TTGATCG82450.030.3024855
TTTGATT468700.029.470174
GTGTTTG2388750.029.468685
GATTTTT181250.029.4273097
TTTGATC276750.028.9884644
TGATTCC92300.028.972766
CTTCCGA22000.028.7152184
TTGATTG71600.028.592195
TTGAGGG201550.027.4531339