FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Mock_30min_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Mock_30min_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9165939
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210050.229163645972333No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201090.21938832453499854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT324800.071.747921
TTTGTGT717050.046.9667662
CTTTGTG721450.046.6836741
TGTGTTT828500.042.5285954
TTGTGTT914050.038.5531433
GTGTTTG1250750.033.2029651
GTTTGAT712300.030.0678023
GATTTTT241100.027.1650897
TTTGATT462200.027.0895774
TTTGAGT214100.024.8476723
TGATTTT275300.024.7074726
TGTTTGA1688000.024.6504882
TTGATTT316950.024.532935
TTTGATC171100.024.3187274
GTTTGAC345950.023.7941193
TTGAGTC134150.023.546034
TTTGACG96900.023.4310044
TGAGTCG46100.023.3892565
CTTGTGT57950.023.2787022
TGAGTCC55350.023.0848035
Produced by FastQC (version 0.11.5)