FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Mock_24h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Mock_24h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8942767
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT84850.039.250321
ACTTTGT150550.022.021153
CTTCCGA26000.019.7293764
TTTGTGT170800.019.2715035
AACTTTG179600.018.9356122
TTCCGAT28550.017.8011445
TGTTTGA399600.016.0384941
CTTTGTG219950.015.1807864
GTGTTTG268550.015.1221512
TCTTCCG34550.015.1220263
TGTGTTT272950.014.8471471
GTTTGAC88100.014.7722722
TTTGAGT77700.012.7758743
TTGTGTT330300.010.8857296
GTTTGAG291450.010.8212312
CCGATCT48300.010.8163167
GAGTCCC16900.010.6798986
TTGAGTC53150.010.3661544
TTGAGCG23950.09.1225294
TTGAGGG42350.08.9722124
Produced by FastQC (version 0.11.5)