FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Mock_24h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Mock_24h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10135127
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344560.3399661395461547No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202450.19975082699999713No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139720.13785717732002767No Hit
ACTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110910.10943128783684705No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103510.10212994864297212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT452250.061.7589341
CTTTGTG726750.038.6739041
TTTGTGT767850.036.6450272
TGTGTTT919400.032.2261664
TTGTGTT981000.030.1930523
GATTTTT372700.029.1315237
GTTTGAT770800.029.122053
GTGTTTG1251450.028.973421
TTTGATT581050.026.8119344
TGATTTT417300.026.6454436
TTGATTT450950.026.6267785
TAACTTT114250.025.2826331
TGTTTGA1581350.022.992892
ATTTTTT543200.019.9614728
GTTTGAC284100.019.7602588
GTTTGAG668000.019.2041238
TTGAGGG107100.018.093279
AACTTTG164950.017.6228222
TTTGATC209050.016.6086754
TTTGACG86400.016.6012029
Produced by FastQC (version 0.11.5)