FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Mock_14h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Mock_14h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9063399
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111440.12295607861906996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT84850.022.9564691
GTGTTTG247650.018.0679532
TTTGTGT153600.017.1631952
TGTGTTT261000.017.110411
TGTTTGA362150.016.0964161
CTTCCGA31300.015.4790764
GTTTGAC80450.014.2884692
CTTTGTG192250.013.9128191
AACTTTG143600.013.8928482
ACTTTGT150800.013.2633691
TTTGAGT94350.012.8376373
TTGTGTT294800.012.4895651
TTCCGAT40500.011.9631065
GTTTGAG258350.011.8406032
CGTATGC12100.011.58161146-47
TCTTCCG41950.011.4360553
TTGAGGG48550.010.8598284
TATGCCG12450.010.30206848-49
GTATGCC13350.09.96338148-49
CCGATCT54800.09.3603937
Produced by FastQC (version 0.11.5)