FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Mock_14h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Mock_14h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11878062
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357090.30062985022304145No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202030.17008666902058603No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123280.1037879748396666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT467750.071.171081
CTTTGTG886650.039.7591321
TTTGTGT895900.039.398332
TGTGTTT1053400.034.9857834
GTGTTTG1594450.033.5396421
GTTTGAT883850.032.626053
TTGTGTT1164250.031.6998793
GATTTTT305550.031.4919667
TTTGATT586800.030.5323854
TTGATTT394750.028.8057615
TGATTTT352950.028.0723296
TAACTTT111600.026.6498851
TGTTTGA2059550.025.9967332
TTTGATC227150.024.7999674
TGATTCC100300.023.6780766
GTTTGAC410600.022.7661153
ATTTTTT425800.022.431028
TTGATCG69550.022.1271345
CTTCCGA31900.020.9945984
TTTGAGT268450.020.6131953
Produced by FastQC (version 0.11.5)